TABLES

List of primers published for the main SCA loci

(modifed from Table 2 of Sequeiros J, Seneca S and Martindale J: Consensus and controversies in best practices for
molecular genetic testing of the spinocerebellar ataxias. Eur J Hum Genet 18:1173-6, 2010)

ATAXIA GENE PRIMERS SEQUENCES REFERENCES 1
SCA1 ATXN1 Rep-1: AACTGGAAATGTGGACGTAC
Rep-2: CAACATGGGCAGTCTGAG

CAG-a: CCGGAGCCCTGCTGAGGT
CAG-b: CCAGACGCCGGGACAC 		(Orr et al, 1993)


(Orr et al, 1993)
DRPLA ATN1 CTGB37.5 F: CACCAGTCTCAACACATCACCATC
CTGB37.5 R: CCTCCAGTGGGTGGGGAAATGCTC

DRPLA F: CCCAGTCCACCGCCCACCCACCA
DRPLA R: TGCTCCAGGAGGAGGGGGCCCAGA 		(Li et al, 1993)


(Majounie et al, 2007)
SCA2 ATXN2 SCA2-A: GGGCCCCTCACCATGTCG
SCA2-B: CGGGCTTGCGGACATTGG

UH13: CGTGCGAGCCGGTGTATGGG
UH10: GGCGACGCTAGAAGGCCGCT 		(Pulst et al, 1996)


(Imbert et al, 1996)
MJD/SCA3 ATXN3 MJD52: CCAGTGACTACTTTGATTCG
MJD25: TGGCCTTTCACATGGATGTGAA

SCA3F: TTTTTAAATATACTTCACTTTTGAATG
SCA3R: TGTGAACTCTGTCCTGATAGGT

SCA3Fanch: GAATGTTTCAGACAGCAGCAAAAGCAG (FAM-labelled)
used with the MJD25 reverse primer (Kawaguchi et al, 1994) 		(Kawaguchi et al, 1994)


(Juvonen et al, 2005)


(Martindale, unpublished)
SCA6 CACNA1A S-5-F1: CACGTGTCCTATTCCCCTGTGATCC
S-5-R1: TGGGTACCTCCGAGGGCCGCTGGTG 		(Zhuchenko et al, 1997)
SCA7 ATXN7 4U1024: TGTTACATTGTAGGAGCGGAA 2
4U716: CACGACTGTCCCAGCATCACTT

H2: GTAGGAGCGGAAAGAATG
H1: TTCAGGACTGGGCAGAGG

SCA7F: CGGCGGCGGGCGGAGCAGCG
SCA7R: TGCGCCGTGGCGGCGGTGGCG

SCA7F: ATGTCGGAGCGGGCCGCGGATGACGTCAGG (label with FAM)
SCA7R: GGAGGCGGCGGCTGCTGCTGCTGCTGCTGC 		(David et al, 1997)


(Del-Favero et al, 1998)


(Juvonen et al, 2005)


(Martindale, unpublished)
SCA8 ATXN8 SCA8-F3: TTTGAGAAAGGCTTGTGAGGACTGAGAATG
SCA8-R4: GGTCCTTCATGTTAGAAAACCTGGCT

SCA8 F: GCAGTATGAGGAAGTGTGGAAA
SCA8 R: GGTCCTTCATGTTAGAAAACCT 		(Koob et al, 1999)


(Majounie et al, 2007)
SCA10 ATXN10 attct-L: AGAAAACAGATGGCAGAATGA
attct-R: GCCTGGGCAACATAGAGAGA

SCA10 F: CTCCAGTGCAACCACTTTTAGA
SCA 10 R: AGGCAGGAGAATTGCTTGAA 		(Matsuura et al, 2000)


(Majounie et al, 2007)
SCA12 PPP2R2B A: TGCTGGGAAAGAGTCGTG
B: GCCAGCGCACTCACCCTC

SCA12 F: TGCTGGGAAAGAGTCGTG
SCA12 R: CAGCGCACTCACCCTCAC 		(Holmes et al, 1999)


(Majounie et al, 2007)
SCA17 TBP TBP-F: CCTTATGGCACTGGACTGAC
TBP-R: GTTCCCTGTGTTGCCTGCTG

SCA17F: AACACCAATAGTCTGTCTATTTTG
SCA17R: TGCTGGGACGTTGACTGCTG

SCA 17F: GACCCCACAGCCTATTCAGA
SCA 17R: GGGACGTTGACTGCTGAAC 		(Koide et al, 1999)


(Juvonen et al, 2005)


(Majounie et al, 2007)

1 Full references are available at: http://www.scabase.eu
2 A SNP at the underlined nucleotide has been reported to EMQN (by Ottie O'Brien, Newcastle, UK)